GeneTex

  • Country / Location Selection

United States (US)

Streptavidin (HRP)

Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403

Cat. No. GTX27403

Reference 2

Application

 WB, IHC-P, IHC-Fr, Dot, ELISA, IM
Package
500 μg ($179)
See all Streptavidin products
PRODUCT

Summary

Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10,000-1:40,000
IHC-P 1:1,000-1:5,000
IHC-Fr Assay dependent
Dot Assay dependent
ELISA 1:20,000-1:200,000
IM Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate (pH7.2), 0.15M Sodium Chloride

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Expression System

Streptomyces avidinii

Purification


This product was prepared from chromatographically purified streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Streptavidin (HRP). GTX27403

GTX27403 ELISA Image

Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)

Streptavidin (HRP). GTX27403

GTX27403 WB Image

WB analysis of human IL-7 protein using Human IL-7 Biotin conjugated antibody followed by GTX27403 Streptavidin (HRP).
Loading : 50 ng
Dilution : 1:1000

Streptavidin (HRP). GTX27403

GTX27403 WB Image

Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)

Streptavidin (HRP). GTX27403

GTX27403 WB Image

WB analysis of GST protein using biotin-conjugated anti-GST antibody followed by GTX27403 Streptavidin (HRP).
Loading : 0.05 μg
Dilution : 1:40000

Streptavidin (HRP). GTX27403

GTX27403 Dot Image

Dot blot analysis of BSA with (row A) and without (row B) biotin conjugation using GTX27403 Streptavidin (HRP).
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

Streptavidin (HRP). GTX27403

GTX27403 WB Image

WB analysis of human IL-7 protein using biontin-conjugated anti-IL-7 antibody followed by GTX27403 Streptavidin (HRP).
Loading : 50 ng
Dilution : 1:40000

REFERENCE

Application Reference

de Souza TL et al. Sci Rep 2019; 9 (1) : 12962 Pro-Cellular Exhaustion Markers are Associated with Splenic Microarchitecture Disorganization and Parasite Load in Dogs with Visceral Leishmaniasis.
Chu PW et al. Fertil Steril 2011; 96 (5) : 1225-9 Notch 1 signaling pathway effect on implantation competency.
Submit a Reference

REVIEW

There are currently no reviews for Streptavidin (HRP) (GTX27403). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 179